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| Status |
Public on Sep 09, 2015 |
| Title |
Homo sapiens gastric carcinoma cells SGC-7901_MTA2 siRNA total |
| Sample type |
RNA |
| |
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| Source name |
Homo sapiens gastric carcinoma SGC-7901
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| Organism |
Homo sapiens |
| Characteristics |
genotype: transfected with siRNA against MTA2(Total; MTA2-homo-556 Sense：5'-3' CAGCCUGGCUGAUAGUAAUTT, Antisense：5'-3' AUUACUAUCAGCCAGGCUGTT; MTA2-homo-2364;Sense：5'-3' GCACCAAUGAGCCUAUUGUTT, Antisense：5'-3' ACAAUAGGCUCAUUGGUGCTT;MTA2-homo-409 Sense：5'-3' GGUGGGAGAUUACGUCUAUTT Antisense：5'-3' AUAGACGUAAUCUCCCACCTT
phenotype: MTA2 knock down
cell line: SGC-7901
cell type: gastric cancer
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| Treatment protocol |
Homo sapiens gastric carcinoma SGC-7901 was grown to Logarithmic phase phase (5% CO2,at 37°C ) in RPMI1640 (Gibco, Eggenstein, Germany) supplemented with 10% (vol) fetal bovine serum (10106078, Gibco) and 1% (vol) penicillin (15140114, Gibco) ,transfected with siRNA negative control or total siRNAs against MTA2 for 48h, or transfected with vector or SNHG5 plasmid
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| Growth protocol |
Homo sapiens gastric carcinoma SGC-7901 was grown to Logarithmic phase phase (5% CO2,at 37°C ) in RPMI1640 (Gibco, Eggenstein, Germany) supplemented with 10% (vol) fetal bovine serum (10106078, Gibco) and 100U/ml penicillin,transfected with siRNA control 20nmol/ml for 48 h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). Total RNA from each sample was quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Total RNA of each sample was used for labeling and array hybridization as the following steps: 1) Reverse transcription with by Invitrogen Superscript ds-cDNA synthesis kit; 2) ds-cDNA labeling with NimbleGen one-color DNA labeling kit; 3) Array hybridization using the NimbleGen Hybridization System and followed by washing with the NimbleGen wash buffer kit; 4) Array scanning using the Axon GenePix 4000B microarray scanner (Molecular Devices Corporation).
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| |
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| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
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| Scan protocol |
Array scanning using the Axon GenePix 4000B microarray scanner (Molecular Devices Corporation).
|
| Description |
This sample is of Homo sapiens gastric carcinoma SGC-7901 transfected with siRNA agianst MTA2 (MTA2-homo-556: Sense：5'-3' CAGCCUGGCUGAUAGUAAUTT, Antisense：5'-3' AUUACUAUCAGCCAGGCUGTT; MTA2-homo-2364:Sense：5'-3' GCACCAAUGAGCCUAUUGUTT, Antisense：5'-3' ACAAUAGGCUCAUUGGUGCTT; MTA2-homo-409:Sense：5'-3' GGUGGGAGAUUACGUCUAUTT, Antisense：5'-3' AUAGACGUAAUCUCCCACCTT)
|
| Data processing |
Scanned images (TIFF format) were then imported into NimbleScan software (version 2.5) for grid alignment and expression data analysis.
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| Submission date |
Feb 13, 2015 |
| Last update date |
Sep 09, 2015 |
| Contact name |
Shi Juan |
| E-mail(s) |
shijuantt@163.com
|
| Phone |
8610-69156430
|
| Organization name |
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
5 Dong Dan San Tiao
|
| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
| |
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| Platform ID |
GPL18943 |
| Series (1) |
| GSE65915 |
Expression analysis of gene expression changes in Homo sapiens SGC-7901 cells after knock down of MTA2 or overexpression of SNHG5 |
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