|
| Status |
Public on Mar 25, 2015 |
| Title |
SeV1.5 24h rep4 |
| Sample type |
RNA |
| |
|
| Source name |
U937_SeV1.5 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U937
cell type: monocytoid
infected with: 1.5 HA u/mL of SeV
|
| Treatment protocol |
Cells were left uninfected or infected with either 1.5 or 150 HA u/mL of SeV for 24 h
|
| Growth protocol |
Cells were grown in RPMI 1640 media + 10% FBS at a concentration of 5 x 10^5 cells/mL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Rneasy minikit (Qiagen) according to the manufacturer's instructions with a DNAse cleanup step
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA was hybridized to the Illumina HumanHT-12 Expression BeadChip
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina hybridization protocol
|
| Description |
rep 4
_9967453010_J
|
| Data processing |
Data extraction was performed using Illumina Genome Studio software without background subtraction or normalization. Signal levels for each array were normalized using quantile normalization after transforming to log base 2 (JMP/Genomics v7.0). Please note that the probe sets with no detectable signal were exclued from the data analysis, resulting a reduced data set of 28372 rows.
|
| |
|
| Submission date |
Mar 24, 2015 |
| Last update date |
Mar 25, 2015 |
| Contact name |
Luna A Zaritsky |
| Organization name |
NIH
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE67198 |
The effect of Sendai virus infection using 2 concentrations on U937 cells |
|
