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Sample GSM16827 Query DataSets for GSM16827
Status Public on Feb 18, 2004
Title 1hHypo_5hReox_4
Sample type RNA
 
Channel 1
Source name HUVEC with 1 hour hypoxia and 5 hours reoxygenation
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Human universal reference RNA (Stratagene, La Jolla, CA)
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description Human umbilical vein endothelial cells (HUVEC) were cultivated on 1%-gelatin-coated 75-cm2 culture flasks in Medium 200 supplemented with 2% fetal calf serum, penicillin (100 units/ml), streptomycin (100 units/ml) and Funizone (0.25 μg/ml) supplied by Cascade Biologics (Portland, OR). Prior to an experiment, HUVECs were subcultivated with Trypsin/EDTA onto 1% gelatin coated glass 30-mm glass coverslips in 60 mm glass Petri dishes.
 
Submission date Feb 12, 2004
Last update date Sep 08, 2006
Contact name Phillip Bowman
E-mail(s) phillip.bowman@cen.amedd.army.mil
Phone 210-916-4297
Organization name Institute of Surgical Research
Street address 3400 Rawley E. Chambers Ave
City San Antonio
State/province TX
ZIP/Postal code 78234-6315
Country USA
 
Platform ID GPL981
Series (1)
GSE1041 Responses of HUVEC to Hypoxia and Reoxygenation

Data table header descriptions
ID_REF
VALUE same as UNF_VALUE but with flagged values removed
F635_MEDIAN Cy5 channel median intensity of the spot.
F635_MEAN Cy5 channel average intensity of the spot.
F635_SD The standard deviation calculated for the Cy5 channel mean intensity of the spot.
B635_MEDIAN Cy5 channel background median intensity around the spot.
B635_MEAN Cy5 channel background average intensity around the spot.
B635_SD The standard deviation calculated for the Cy5 channel background average intensity around the spot.
F635_QUALITY Cy5 channel percentage of pixels in the spot more than one standard deviation above background.
F532_MEDIAN Cy3 channel median intensity of the spot.
F532_MEAN Cy3 channel average intensity of the spot.
F532_SD The standard deviation calculated for the Cy3 channel mean intensity of the spot.
B532_MEDIAN Cy3 channel background median intensity around the spot.
B532_MEAN Cy3 channel background average intensity around the spot.
B532_SD The standard deviation calculated for the Cy3 channel background average intensity around the spot.
F532_QUALITY Cy3 channel percentage of pixels in the spot more than one standard deviation above background.
FLAGS The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity.
UNF_VALUE log ratio of Median of Ratios between Cy5 and Cy3 channels. (log2 of Median of Ratios). The Median of Ratios is the median of the pixel-by-pixel ratios of pixel intensities that have had the median background intensity subtracted.

Data table
ID_REF VALUE F635_MEDIAN F635_MEAN F635_SD B635_MEDIAN B635_MEAN B635_SD F635_QUALITY F532_MEDIAN F532_MEAN F532_SD B532_MEDIAN B532_MEAN B532_SD F532_QUALITY FLAGS UNF_VALUE
1 0.546259584 528 566 314 495 548 314 15 124 134 53 113 137 141 3 -50 0.546259584
2 1.592611785 434 476 259 467 493 282 17 123 126 40 118 129 63 7 -50 1.592611785
3 2.006765318 455 486 268 430 510 632 1 120 119 24 110 116 42 9 -50 2.006765318
4 -3.137245682 1193 1265 510 411 461 270 88 2649 2745 643 103 115 70 100 0 -3.137245682
5 -2.512634105 959 1017 382 408 459 286 82 1316 1302 286 109 144 181 96 0 -2.512634105
6 2.022969194 516 677 427 454 520 322 32 130 136 42 118 151 173 1 -50 2.022969194
7 -2.704451712 2252 2301 923 441 499 299 98 4281 4285 1058 118 126 45 100 0 -2.704451712
8 -1.870865248 1011 1034 451 428 478 298 74 869 883 327 108 113 34 99 0 -1.870865248
9 -1.200006252 1117 1858 2506 431 501 493 71 705 1528 4102 103 118 88 100 0 -1.200006252
10 -0.088391915 1233 1322 838 515 563 329 66 436 675 714 140 194 208 66 0 -0.088391915
11 1.559926322 1979 2280 1636 442 530 697 95 297 491 1251 109 181 1260 3 0 1.559926322
12 1.733767877 892 896 379 508 588 381 50 171 202 145 123 131 45 56 0 1.733767877
13 -2.724443187 567 634 349 441 546 930 2 733 787 578 106 219 2029 0 0 -2.724443187
14 0.364203323 430 707 582 481 502 248 41 334 340 47 146 199 118 100 0 0.364203323
15 -0.850584217 497 555 279 456 487 291 26 304 293 168 121 181 127 61 -50 -0.850584217
16 -0.797561116 570 600 302 434 481 261 26 298 275 120 112 168 119 67 -50 -0.797561116
17 -0.469821021 504 541 258 480 524 303 19 175 225 127 114 173 131 46 -50 -0.469821021
18 -1.006049699 629 620 323 459 500 287 28 308 291 124 113 167 116 73 -50 -1.006049699
19 0.323403572 448 480 251 452 486 268 13 164 167 57 114 142 78 36 -50 0.323403572
20 0.472441958 489 550 343 418 471 271 25 198 184 70 115 137 67 55 -50 0.472441958

Total number of rows: 23232

Table truncated, full table size 1963 Kbytes.




Supplementary data files not provided
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