|
Status |
Public on Dec 17, 2015 |
Title |
T47D_PRnull_vehicle_3 |
Sample type |
RNA |
|
|
Source name |
Breast T47D cell line, progesterone receptor null, vehicle treated 24 hr
|
Organism |
Homo sapiens |
Characteristics |
parental cell line: T47D-Y parental cell type: breast cancer cell line stable vector integration: none treatment: ethanol vehicle time point: 24 hr
|
Treatment protocol |
Samples treated in IMEM medium plus 5% DCC, with or without 10e-8 M R5020 for 24 hr.
|
Growth protocol |
Samples grown in complete medium (MEM, 5% FBS, 1% NEAA, 1% pen/strep, 6ng/ml insulin, 200 ug/ml G418) before 1 day starvation in IMEM medium plus 5% DCC, then treated in IMEM medium plus 5% DCC for 24 hr.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
T47D_NULL_V_3 Replicate 3
|
Data processing |
The data were filtered and any probes with a detection P value less than 0.05 were removed from the normalized dataset. The data was then normalized using log2 transformation and quantile normalization with R using the lumi bioconductor package.
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|
|
Submission date |
May 27, 2015 |
Last update date |
Dec 17, 2015 |
Contact name |
Todd P Knutson |
E-mail(s) |
knut0297@umn.edu
|
Phone |
612-626-8911
|
Organization name |
University of Minnesota
|
Department |
Minnesota Supercomputing Institute
|
Street address |
117 Pleasant St SE
|
City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE69295 |
Active FOXO1 is a Key Determinant of Isoform-Specific Progesterone Receptor Transactivation and Senescence Programming |
GSE69296 |
Active FOXO1 is a Key Determinant of Isoform-Specific Progesterone Receptor Transactivation and Senescence Programming |
|