|
| Status |
Public on Dec 17, 2015 |
| Title |
T47D_PRB_R5020_3 |
| Sample type |
RNA |
| |
|
| Source name |
Breast T47D cell line, progesterone receptor isoform B expressing, R5020 treated 24 hr
|
| Organism |
Homo sapiens |
| Characteristics |
parental cell line: T47D-Y
parental cell type: breast cancer cell line
stable vector integration: pSG5-PRB
treatment: R5020
time point: 24 hr
|
| Treatment protocol |
Samples treated in IMEM medium plus 5% DCC, with or without 10e-8 M R5020 for 24 hr.
|
| Growth protocol |
Samples grown in complete medium (MEM, 5% FBS, 1% NEAA, 1% pen/strep, 6ng/ml insulin, 200 ug/ml G418) before 1 day starvation in IMEM medium plus 5% DCC, then treated in IMEM medium plus 5% DCC for 24 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
T47D_PRB_R_3
Replicate 3
|
| Data processing |
The data were filtered and any probes with a detection P value less than 0.05 were removed from the normalized dataset. The data was then normalized using log2 transformation and quantile normalization with R using the lumi bioconductor package.
|
| |
|
| Submission date |
May 27, 2015 |
| Last update date |
Dec 17, 2015 |
| Contact name |
Todd P Knutson |
| E-mail(s) |
knut0297@umn.edu
|
| Phone |
612-626-8911
|
| Organization name |
University of Minnesota
|
| Department |
Minnesota Supercomputing Institute
|
| Street address |
117 Pleasant St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE69295 |
Active FOXO1 is a Key Determinant of Isoform-Specific Progesterone Receptor Transactivation and Senescence Programming |
| GSE69296 |
Active FOXO1 is a Key Determinant of Isoform-Specific Progesterone Receptor Transactivation and Senescence Programming |
|
