|
| Status |
Public on Apr 01, 2007 |
| Title |
Normal Bone Marrow 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Case 47
|
| Organism |
Homo sapiens |
| Characteristics |
n/a
|
| Biomaterial provider |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by purification using RNeasy
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
RNA pooled from ten human cell lines
|
| Biomaterial provider |
Stratagene
|
| Extracted molecule |
total RNA |
| Extraction protocol |
None
|
| Label |
Cy5
|
| Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
| |
|
| |
| Hybridization protocol |
Arrays were hybridized and washed as described in Andersson et al et al. (Leukemia 2005)
|
| Scan protocol |
Arrays were scanned in an Agilent Microarray scanner
|
| Description |
cDNA microarray data
|
| Data processing |
Image analysis was performed using Genepix v4.0. Resultsfile were uploaded into BASE where they were filtered, normalized using pin-based lowess, mean centered with respect to hospital and filtered for presence (40% required)
|
| |
|
| Submission date |
Mar 01, 2007 |
| Last update date |
Mar 05, 2007 |
| Contact name |
Thoas, Anna Fioretos, Andersson |
| E-mail(s) |
thoas.fioretos@med.lu.se, anna.andersson@med.lu.se
|
| Organization name |
Dept of Clinical Genetics
|
| Street address |
Lund University Hospital
|
| City |
Lund |
| ZIP/Postal code |
22185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL4861 |
| Series (1) |
| GSE7186 |
Microarray-based classification of a consecutive series of 121 childhood acute leukemias |
|
