|
Status |
Public on Apr 01, 2007 |
Title |
AML 10 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Case 60
|
Organism |
Homo sapiens |
Characteristics |
11q23/MLL
|
Biomaterial provider |
None
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction followed by purification using RNeasy
|
Label |
Cy3
|
Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
|
|
Channel 2 |
Source name |
Stratagene Universal Human Reference RNA
|
Organism |
Homo sapiens |
Characteristics |
RNA pooled from ten human cell lines
|
Biomaterial provider |
Stratagene
|
Extracted molecule |
total RNA |
Extraction protocol |
None
|
Label |
Cy5
|
Label protocol |
Samples were labeled using the CyScribe Post-labeling Kit (Amersham Biosciences)
|
|
|
|
Hybridization protocol |
Arrays were hybridized and washed as described in Andersson et al et al. (Leukemia 2005)
|
Scan protocol |
Arrays were scanned in an Agilent Microarray scanner
|
Description |
cDNA microarray data
|
Data processing |
Image analysis was performed using Genepix v4.0. Resultsfile were uploaded into BASE where they were filtered, normalized using pin-based lowess, mean centered with respect to hospital and filtered for presence (40% required)
|
|
|
Submission date |
Mar 01, 2007 |
Last update date |
Mar 05, 2007 |
Contact name |
Thoas, Anna Fioretos, Andersson |
E-mail(s) |
thoas.fioretos@med.lu.se, anna.andersson@med.lu.se
|
Organization name |
Dept of Clinical Genetics
|
Street address |
Lund University Hospital
|
City |
Lund |
ZIP/Postal code |
22185 |
Country |
Sweden |
|
|
Platform ID |
GPL4861 |
Series (1) |
GSE7186 |
Microarray-based classification of a consecutive series of 121 childhood acute leukemias |
|