|
| Status |
Public on Apr 09, 2007 |
| Title |
Spinal Cord 9 Normal |
| Sample type |
RNA |
| |
|
| Source name |
Male/Female; Normal/Diseased
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue/Cell Line [C]: spinal_cord
Disease/Normal or Treatment [C]: normal
Gender: F
Disease type: Normal
|
| Treatment protocol |
Not treated
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Normal
|
| Data processing |
The data were analyzed with robust multi-array (RMA) for background correction, normalization and polishing.
|
| |
|
| Submission date |
Mar 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Richard B Roth |
| E-mail(s) |
rroth@neurocrine.com
|
| Organization name |
Neurocrine Biosciences, Inc.
|
| Department |
Molecular Medicine
|
| Street address |
12790 El Camino Real
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92130 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7307 |
Human body index - transcriptional profiling |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
