Department of Neurology, School of Medicine, University of São Paulo, Brazil
Treatment protocol
Tumor specimens were obtained from patients with CNS tumors treated by the Neurosurgery Group of the Department of Neurology at Hospital das Clínicas, School of Medicine, University of São Paulo, São Paulo, Brazil, between 2000 and 2005. The tissues were categorized according to the WHO grading system (PMID: 11895036, PMID: 8047695) by neuropathologists from the Division of Pathological Anatomy of the same institution. Informed consent was obtained from each patient. Fresh surgical samples of different grades and non-neoplastic tissues of the CNS (temporal lobectomy from epilepsy surgeries) were macrodissected and immediately snap-frozen in liquid nitrogen upon surgical removal.
Extracted molecule
total RNA
Extraction protocol
All tissues were microdissected and RNA was extracted with trizol.
Label
Cy5
Label protocol
As described in the manufacturer's protocol.
Hybridization protocol
As described in the manufacturer's protocol.
Scan protocol
Fluorescence was measured using an Axon GenePix 4000B Scanner (Axon Instruments Inc, Sunnyvale, CA).
Description
na
Data processing
Data were normalized with the CodeLink Software v.2.3 (GE Healthcare, Piscataway, NJ).
