|
| Status |
Public on Apr 01, 2007 |
| Title |
spt3 del in Y13.2 deriv w/ p2uLF-T(K145E V161E) vs. WT Y13.2 w/ p2uLF-T(M1Stop) (5-018A-670700) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
spt3(delta)_p2uLF-T(K145E V161E)(Gal10)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
spt3 deletion in Y13.2 derivative strain transformed with p2uLF-T(K145E V161E)(Gal10) plasmid
|
| Biomaterial provider |
Pugh Lab, Penn State University
|
| Treatment protocol |
Galactose induction (see below)
|
| Growth protocol |
Transformants carrying the TBP plasmids were grown in CSM-Leu-Trp + 3% raffinose liquid media until they reached an OD600 = 0.65-0.8. Expression of the TBP was induced by addition of galactose to a final concentration of 2%.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| Label |
Cy5
|
| Label protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| |
|
| Channel 2 |
| Source name |
WT_p2uLF-T(M1Stop)(Gal10)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
WT Y13.2 derivative strain transformed with p2uLF-T(M1Stop)(Gal10) plasmid
|
| Biomaterial provider |
Pugh Lab, Penn State University
|
| Treatment protocol |
Galactose induction (see below)
|
| Growth protocol |
Transformants carrying the TBP plasmids were grown in CSM-Leu-Trp + 3% raffinose liquid media until they reached an OD600 = 0.65-0.8. Expression of the TBP was induced by addition of galactose to a final concentration of 2%.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| Label |
Cy3
|
| Label protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| |
|
| |
| Hybridization protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| Scan protocol |
Chitikila et al. 2002. Mol Cell. 10:871-82
|
| Description |
one of at least 2 independent biological replicates, Test/Ref with Ref=WT strain with M1stop mutant
|
| Data processing |
MeanFRG- MedianBCK>S.D. BCK, <26% saturation. Log2 Ratio of Test/Ref; see publication for normalized data
|
| |
|
| Submission date |
Mar 28, 2007 |
| Last update date |
Mar 29, 2007 |
| Contact name |
B. Franklin Pugh |
| E-mail(s) |
fp265@cornell.edu, bfp2@psu.edu
|
| Organization name |
Cornell University
|
| Department |
Molecular Biology and Genetics
|
| Lab |
B. Franklin Pugh
|
| Street address |
465 Biotechnology Bldg.
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL1220 |
| Series (1) |
| GSE7385 |
A TATA binding protein regulatory network |
|
