|
| Status |
Public on Nov 04, 2007 |
| Title |
slide 15- 11 cy3 untreated cy 5 HDAC7-VP16 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
DO11.10 T cell hybridoma, untreated
|
| Organism |
Mus musculus |
| Characteristics |
unmanipulated cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAsol total RNA extraction using acid phenol and guanidinium isothiocyanate, per Current Protocols in Molecular Biology
|
| Label |
cy3
|
| Label protocol |
Incorporation of aminoallyl-dUTP during cDNA first strand synthesis, followed by alkaline coupling to NHS-Cy3 and NHS-Cy5
|
| |
|
| Channel 2 |
| Source name |
DO11.10 T cell hybridoma, transduced with HDAC7-VP16
|
| Organism |
Mus musculus |
| Characteristics |
cells spin-infected with MSCV IRES GFP retrovirus encoding activating HDAC7-VP16 fusion protein
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAsol total RNA extraction using acid phenol and guanidinium isothiocyanate, per Current Protocols in Molecular Biology
|
| Label |
cy3
|
| Label protocol |
Incorporation of aminoallyl-dUTP during cDNA first strand synthesis, followed by alkaline coupling to NHS-Cy3 and NHS-Cy5
|
| |
|
| |
| Hybridization protocol |
Ambion Gene TAC Hybstation. O/N at 42 degC in Ambion SlideHyb 2 buffer, 20%formamide, wash 42 degC in 2X SSC
|
| Scan protocol |
Axon 4000B scanner, data acquired with GenePix 6.0 software
|
| Description |
untreated vs. HDAC7-VP16: 10 replicates
|
| Data processing |
LOWESS and print-tip normalizations, median pixel values used, no background subtraction
|
| |
|
| Submission date |
Apr 05, 2007 |
| Last update date |
Jun 08, 2007 |
| Contact name |
Eric Verdin |
| E-mail(s) |
everdin@buckinstitute.org
|
| Phone |
(415) 209-2250
|
| Organization name |
Buck Institute for Research on Aging
|
| Department |
NA
|
| Lab |
Verdin
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL5064 |
| Series (1) |
| GSE7468 |
Gene Regulation by HDAC7 in Thymic Selection |
|
