CD4+ T cells were isolated from blood samples by using RosetteSep CD4+ enrichment kit (StemCell Technologies, Meylan, France); purity: > 90% as determined by flow cytometry. RNA was isolated with Trizol(r) method according to the manufacturer's protocol. The RNA was purified with the RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany).
Label
biotin
Label protocol
Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
Hybridization protocol
Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol
Scanning was performed on the Illumina(r) BeadStation 500x.
