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Sample GSM182321 Query DataSets for GSM182321
Status Public on Apr 01, 2008
Title Self-Hyb_mature-leaf
Sample type RNA
 
Channel 1
Source name mature leaf
Organism Oryza sativa Japonica Group
Characteristics cultivar: Nipponbare
Tissue: flag leaf
stage: booting stage
Growth protocol growth: soil environment
growth enviroment: field study
light condition: not controlled
Extracted molecule total RNA
Extraction protocol Separation technique: Trimming
Extraction source: Frozen
Kit: RNeasy Midi Kit
Manufacturer: QIAGEN
Manual: QIAGEN
Kit: OligotexTM-dt30 <Super> mRNA Purification kit (From Total RNA)
Manufacturer: TaKaRa
Manual: TaKaRa
Label Cy3
Label protocol Kit: Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Linear Amplification kit standard protocol
 
Channel 2
Source name mature leaf
Organism Oryza sativa Japonica Group
Characteristics cultivar: Nipponbare
Tissue: flag leaf
stage: booting stage
Growth protocol growth: soil environment
growth enviroment: field study
light condition: not controlled
Extracted molecule total RNA
Extraction protocol Separation technique: Trimming
Extraction source: Frozen
Kit: RNeasy Midi Kit
Manufacturer: QIAGEN
Manual: QIAGEN
Kit: OligotexTM-dt30 <Super> mRNA Purification kit (From Total RNA)
Manufacturer: TaKaRa
Manual: TaKaRa
Label Cy5
Label protocol Kit: Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Linear Amplification kit standard protocol
 
 
Hybridization protocol hybridization_Kit: in situ hybridization plus
hybridization_Manufacturer: Agilent Technologies
hybridization_Manual: Agilent Technologies
Scan protocol Scanner: Agilent DNA microarray Scaner
Manufacturer: Agilent Technologies
Software: Scanner Control software
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
Description self-hybridization
Data processing Software: Feature Extraction ver 8.1.1.1
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
 
Submission date Apr 16, 2007
Last update date Apr 16, 2007
Contact name Kouji Satoh
Organization name NARO/HARC
Street address Hitsujigaoka 1
City Sapporo
State/province Hokkaido
ZIP/Postal code 062-8555
Country Japan
 
Platform ID GPL477
Series (1)
GSE7530 Gene expression profiles of various tissues under different physiological conditions

Data table header descriptions
ID_REF
VALUE log10 ratio (Ch2 normalized signal intensity / Ch1 normalized signal intensity)
PValueLogRatio Significance level of the Log Ratio computed for a feature
gBGSubSignal The net CH1 signal following the subtraction of the background from the raw mean CH1 signal
rBGSubSignal The net CH2 signal following the subtraction of the background from the raw mean CH2 signal

Data table
ID_REF VALUE PValueLogRatio gBGSubSignal rBGSubSignal
1 -3.561425468 7.83691E-36 16737.8 8.22781
2 -3.269747202 8.14144E-36 26139.1 25.0962
3 1 1.98777 2.39396
4 -0.284463121 0.73804078 9.57596 5.86031
5 -3.807123702 7.68195E-36 19246.9 4.92991
6 -3.275906231 8.13001E-36 27493 26.0312
7 1 6.83015 0.178692
8 1 0.911131 0.503626
9 -3.702282511 7.73292E-36 19430.7 6.90589
10 -3.286967747 8.11266E-36 27775.8 25.6371
11 1 4.67129 0.399427
12 1 3.93806 0.378879
13 -3.727936674 7.72598E-36 17593.5 4.63799
14 -3.247712039 8.1748E-36 28142.5 28.4456
15 1 1.4547 0.988816
16 1 -0.489784 1.45547
17 -3.794291944 7.68926E-36 18767 4.08626
18 -3.3003883 8.09143E-36 28884.7 25.8536
19 1 1.39513 -0.810895
20 1 -2.04081 -2.35583

Total number of rows: 22575

Table truncated, full table size 985 Kbytes.




Supplementary file Size Download File type/resource
GSM182321.txt.gz 4.8 Mb (ftp)(http) TXT

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