|
| Status |
Public on Apr 01, 2008 |
| Title |
dye-swap experiment between primary callus and shoot 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
primary callus
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
cultivar: Nipponbare
Tissues: cultured callus
stage: in-vitro culture stage
age: incubation for 7 days
|
| Growth protocol |
growth: in vitro culture
growth enviroment: growth chamber study
Growth substrates: callus induction medium
light condition: continuous light
temperature: 30 degreeC
reference: Kyozuka and Shimamoto (1991) Transformation and regeneration of rice protoplasts. In K Lindsey, ed, Plant Tissue Culture Manual B1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Separation technique: Trimming
Extraction source: Frozen
Kit: RNeasy Midi Kit
Manufacturer: QIAGEN
Manual: QIAGEN
Kit: OligotexTM-dt30 <Super> mRNA Purification kit (From Total RNA)
Manufacturer: TaKaRa
Manual: TaKaRa
|
| Label |
Cy3
|
| Label protocol |
Kit: Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Linear Amplification kit standard protocol
|
| |
|
| Channel 2 |
| Source name |
10 days old shoot5
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
cultivar: Nipponbare
Tissue: shoot
stage: LP.03 three leaves visible
age: 10 days after sowing
|
| Growth protocol |
growth: hydroponic culture
growth enviroment: growth chamber study
Growth substrates: 1/5000 diluted Hyponex (10-3-3) solution
light condition: 16hrs day length
temperature: 28 degreeC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Separation technique: Trimming
Extraction source: Frozen
Kit: RNeasy Midi Kit
Manufacturer: QIAGEN
Manual: QIAGEN
Kit: OligotexTM-dt30 <Super> mRNA Purification kit (From Total RNA)
Manufacturer: TaKaRa
Manual: TaKaRa
|
| Label |
Cy5
|
| Label protocol |
Kit: Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Linear Amplification kit standard protocol
|
| |
|
| |
| Hybridization protocol |
hybridization_Kit: in situ hybridization plus
hybridization_Manufacturer: Agilent Technologies
hybridization_Manual: Agilent Technologies
|
| Scan protocol |
Scanner: Agilent DNA microarray Scaner
Manufacturer: Agilent Technologies
Software: Scanner Control software
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
|
| Description |
10 days old shoot5' is control sample, and is same as sample of sample name shoot_self5. 'primary callus' is target sample, and is same as sample of sample name Prim_callus_self
|
| Data processing |
Software: Feature Extraction ver 8.1.1.1
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
|
| |
|
| Submission date |
Apr 16, 2007 |
| Last update date |
Apr 16, 2007 |
| Contact name |
Kouji Satoh |
| Organization name |
NARO/HARC
|
| Street address |
Hitsujigaoka 1
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
062-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL477 |
| Series (1) |
| GSE7531 |
Differentially expressed genes from different developmantal stages in rice |
|
