|
| Status |
Public on Jun 10, 2016 |
| Title |
wts149 d5 1b.RNAseq |
| Sample type |
SRA |
| |
|
| Source name |
wing imaginal disc
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: third instar wandering larvae
dissection: Hand dissection
genotype: ubxflp: FRT82B wts149 clones in Minute background
time point: day5
|
| Treatment protocol |
Wandering third instar larvae were collected and wing imaginal discs were isolated by dissection for each genotype on the day indicated (day 5 or 9). Dissection tools and surfaces were prepared with RNAseAway (Ambion.) Discs were dissected from larvae and transferred into RNAse-free 1xPBS on ice.
|
| Growth protocol |
All flies were raised at 25˚C on standards fly food
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNAeasy mini spin kit (Qiagen).
Quality control, library preparation and sequencing were performed by Genomic Technologies Facility at the Center for Integrative Genomics, University of Lausanne.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
wts d5 1b
|
| Data processing |
cleaning (fastq-mcf)
mapping to D. melanogaster genome r 6.03 using TopHat2.0.11 (default parameters)
quantification of gene expression measures with HT-Seq count (option -str=no) using D. melanogaster gene annotation release 6.03 from FlyBase
Genome_build: Drosophila melanogaster FB6
table of counts (From HT-seq)
|
| |
|
| Submission date |
Jul 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Delphine Potier |
| E-mail(s) |
potier@ciml.univ-mrs.fr
|
| Organization name |
CIML
|
| Street address |
163 avenue de luminy
|
| City |
Marseille |
| ZIP/Postal code |
13009 |
| Country |
France |
| |
|
| Platform ID |
GPL17275 |
| Series (2) |
| GSE71353 |
An ectopic network of transcription factors regulated by Hippo signaling drives growth and invasion of a malignant tumor model [larval wild type discs] |
| GSE71354 |
An ectopic network of transcription factors regulated by Hippo signaling drives growth and invasion of a malignant tumor model |
|
| Relations |
| Reanalyzed by |
GSM3276464 |
| BioSample |
SAMN03938673 |
| SRA |
SRX1118815 |
