|
| Status |
Public on Apr 30, 2016 |
| Title |
mdxLG55C 3m |
| Sample type |
RNA |
| |
|
| Source name |
Double mutant
|
| Organism |
Mus musculus |
| Characteristics |
tissue: calf muscles
Sex: male
lineage: Dmdmdx/Largemyd
age: 3m
|
| Treatment protocol |
no treatment
|
| Growth protocol |
The calf muscles were dissected, rapidly frozen in liquid nitrogen and powdered using a pestle and mortar.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. Quantity and quality of RNA was assessed with Nanodrop 8000 Spectrophotometer (Thermo Scientific, Schwerte, Germany), agarose gel electrophoresis and integrity with Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). Only samples with 260/280 ratio greater than 1.8, preserved rRNA 28S/18S ratio and RIN (RNA integrity number) >7.0 were used.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
RMA expression value derived from Expression Console software
|
| Data processing |
Data were analyzed with Expression Console (Affymetrix).Normalization was done with RMA method
|
| |
|
| Submission date |
Aug 18, 2015 |
| Last update date |
Apr 30, 2016 |
| Contact name |
Camila F Almeida |
| E-mail(s) |
camilafreitasalmeida@gmail.com
|
| Organization name |
Nationwide Children's Hospital
|
| Department |
Center for Gene therapy
|
| Street address |
700 Children's Drive, RB2 WA3103
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE72151 |
Transcriptome analysis of Largemyd and Dmdmdx/Largemyd muscles in comparison to Dmdmdx: what make them different? |
|
