|
| Status |
Public on Aug 27, 2015 |
| Title |
IL-22-2 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
IL-22-2 [miRNA]
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HaCaT
agent: IL-22 stimulation
|
| Treatment protocol |
To prepare them for recombinant human IL-22 (Sangon Biological Engineering Technology Company, Shanghai, China) treatment, HaCaT cells were starved in serum-free DMEM for 24 h and then treated with IL-22 (100 ng/ml) in serum-free DMEM for another 24 h or not treated.
|
| Growth protocol |
HaCaT cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS),100 U/ml penicillin and 100µg/ml streptomycin at 37℃ in a humidified atmosphere containing 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 48C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip2 Scanner 3000 7G
|
| Data processing |
GeneChip Command Console Software (AGCC).
|
| |
|
| Submission date |
Aug 26, 2015 |
| Last update date |
Aug 27, 2015 |
| Contact name |
Meng Jiang |
| E-mail(s) |
1037447134@qq.com
|
| Phone |
15066142801
|
| Organization name |
Qilu hospital
|
| Street address |
lixia street
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE72396 |
Expression data from HaCaT cells stimulated by IL-22 |
|
