Severe Acute Respiratory Syndrome (SARS) virus amplified from Vero cell culture as described in Lim PL, Kurup A, Gopalakrishna G, Chan KP, Wong CW, et al: Laboratory-Acquired Severe Acute Respiratory Syndrome. N Engl J Med 2004, 350:1740-1745, PMID: 15103000, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=15103000
Extracted molecule
total RNA
Extraction protocol
RNAzol per manufacturer's instructions
Label
Cy3
Label protocol
Random PCR, end-label with biotin, amplify signal with streptavidin-Cy3 - see Wong et al, 2007
Hybridization protocol
Overnight hybridization - see Wong et al, 2007
Scan protocol
Axon scanners at PMT indicated in the title of data file