 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 17, 2007 |
| Title |
A549 H1-term transfected with Luc duplex versus control transfected A549 H1-term, 24 hours |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mock transfected A549 H1-term, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Mock transfected A549 H1-term, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy3
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| Channel 2 |
| Source name |
A549 H1-term transfected with Luc duplex, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
A549 H1-term transfected with Luc duplex, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy5
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| |
| Hybridization protocol |
Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7, PMID 11283592.
|
| Scan protocol |
Agilent LP2 laser scanner
|
| Description |
Transfection of duplexes into cell lines as described in He et al., Nature, 2007
|
| Data processing |
Error model as described in Hughes et al and in US patent #6,351,712
|
| |
|
| Submission date |
May 21, 2007 |
| Last update date |
Jul 16, 2007 |
| Contact name |
Lee Lim |
| E-mail(s) |
lee_lim@merck.com
|
| Phone |
206-802-7323
|
| Organization name |
Rosetta Inpharmatics
|
| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL4372 |
| Series (1) |
| GSE7864 |
A microRNA component of the p53 tumor suppressor network |
|
| Data table header descriptions |
| ID_REF |
Rosetta generated unique probe identifier |
| VALUE |
LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted] |
| LOG_INTENSITY |
Averaged log10 intensity of a fluor-reversed chip pair |
| P_VALUE |
P value that the probe target is differentially expressed in the two tissues |
| INTENSITY1 |
CHANNEL1 intensity; averaged raw data from a fluor-reversed pair |
| INTENSITY2 |
CHANNEL2 intensity; averaged raw data from a fluor-reversed pair |
| Data table |
| ID_REF |
VALUE |
LOG_INTENSITY |
P_VALUE |
INTENSITY1 |
INTENSITY2 |
| 10019475365 |
0.011934596113860607 |
-0.45880284905433655 |
0.81858384609222412 |
0.34518998861312866 |
0.35385000705718994 |
| 10019481149 |
0.0017175066750496626 |
-0.3360973596572876 |
0.9749494194984436 |
0.46687999367713928 |
0.46529501676559448 |
| 10019495284 |
0.042889665812253952 |
0.45730593800544739 |
0.50379753112792969 |
2.7380950450897217 |
3.01242995262146 |
| 10019687586 |
0.051749594509601593 |
-0.99543452262878418 |
0.42804118990898132 |
0.094944998621940613 |
0.10765999555587769 |
| 10019713746 |
0.020724209025502205 |
-0.26460790634155273 |
0.6364404559135437 |
0.54568499326705933 |
0.57245504856109619 |
| 10019799479 |
0.0048856809735298157 |
-0.65457296371459961 |
0.92336815595626831 |
0.22055500745773315 |
0.22311499714851379 |
| 10019809115 |
-0.0076299998909235001 |
0.11032397300004959 |
0.89203953742980957 |
1.3107750415802002 |
1.2995749711990356 |
| 10019874890 |
0.0064603178761899471 |
-0.57983136177062988 |
0.90951442718505859 |
0.26316499710083008 |
0.26857000589370728 |
| 10019903058 |
0.065775074064731598 |
-1.2012982368469238 |
0.65631729364395142 |
0.062570005655288696 |
0.068190000951290131 |
| 10019909307 |
-0.010079850442707539 |
-1.1529818773269653 |
0.86165046691894531 |
0.074495002627372742 |
0.073339998722076416 |
| 10019911222 |
0.012267500162124634 |
0.67114126682281494 |
0.81209158897399902 |
4.6249551773071289 |
4.7646799087524414 |
| 10019924807 |
0.032829690724611282 |
-1.3143460750579834 |
0.71033209562301636 |
0.046604998409748077 |
0.051030002534389496 |
| 10019927856 |
0.011744840070605278 |
0.6904444694519043 |
0.81872344017028809 |
4.8370752334594727 |
4.9713950157165527 |
| 10019932383 |
0.003297693096101284 |
-0.031143240630626678 |
0.95998293161392212 |
0.93068498373031616 |
0.9345550537109375 |
| 10019948931 |
-0.0043127415701746941 |
-1.1002898216247559 |
0.96407920122146606 |
0.083375006914138794 |
0.081890001893043518 |
| 10019975533 |
0.040340568870306015 |
-1.3640137910842896 |
0.6945536732673645 |
0.042865000665187836 |
0.044294998049736023 |
| 10019977224 |
0.023223087191581726 |
0.45568016171455383 |
0.72509533166885376 |
2.7892451286315918 |
2.9341049194335937 |
| 10019977227 |
0.081869997084140778 |
-0.36979809403419495 |
0.50875675678253174 |
0.38839000463485718 |
0.4689599871635437 |
| 10019987588 |
-0.0063367937691509724 |
-0.90820944309234619 |
0.91601908206939697 |
0.12546999752521515 |
0.1251550018787384 |
| 10020008603 |
0.0074479999020695686 |
-0.76004117727279663 |
0.95202696323394775 |
0.17227999866008759 |
0.17526000738143921 |
Total number of rows: 39301
Table truncated, full table size 4361 Kbytes.
| Supplementary data files not provided |
| Processed data included within Sample table |
|
|
|
|
 |
