|
| Status |
Public on May 24, 2016 |
| Title |
Subject 7 RE |
| Sample type |
RNA |
| |
|
| Source name |
Human Vastus lateralis after resistance exercise, Subject 7
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Vastus lateralis
treatment: Resistance exercise
gender: Male
subject: Subject 7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biopsies were obtained from the vastus lateralis muscle using the Bergström needle technique. The biopsies were immediately stored in liquid nitrogen until processing. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HuGene-2_1-st array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Skeletal muscle after resistance exercise, Subject 7
|
| Data processing |
The probe-set level intensities of the arrays were normalized using the Robust Multi-array Analysis method (RMA) implemented within the R statistical software environment using the 'oligo' package (Bioconductor project).
|
| |
|
| Submission date |
Oct 20, 2015 |
| Last update date |
May 24, 2016 |
| Contact name |
Eric Rullman |
| E-mail(s) |
eric.rullman@gmail.com
|
| Phone |
+46858586777
|
| Organization name |
Karolinska Institutet
|
| Department |
Laboratory Medicine
|
| Lab |
Clinical Physiology
|
| Street address |
C1:82 Karolinska University Hospital, Huddinge
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE74194 |
Muscle Transcriptome Profile of Resistance Exercise is Augmented by Aerobic Exercise |
|
