|
| Status |
Public on Oct 24, 2015 |
| Title |
Leaf 10 8mm primordium |
| Sample type |
RNA |
| |
|
| Source name |
Leaf 10 8mm primordium
|
| Organism |
Zea mays |
| Characteristics |
tissue: leaf
|
| Treatment protocol |
Emerging seedlings of sample 5JA (first leaf partially expanded) received a single application of 100µl of varying concentrations of jasmonic acid to the apical whorl, other samples untreated
|
| Growth protocol |
B73 and Mo17 were crossed to generate hybrid seeds, which were planted and grown in a temperature-controlled greenhouse and illuminated for 14 hours daily under 1kW metal halide and sodium lights (www.osram.com). Plants were grown in Compost Plus growing mix (www.beautifullandproducts.com).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Leaf primordia were isolated by dissection of shoot tips and flash-frozen in liquid nitrogen. Total RNA was extracted by Trizol (www.lifetechnologies.com) and purified on RNeasy columns (www.qiagen.com) using the manufacturer’s protocols.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to the manufacturer's protocol, see www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufacturer's protocol, see www.nimblegen.com.
|
| Scan protocol |
Scanning was performed according to the manufacturer's protocol, see www.nimblegen.com.
|
| Data processing |
Array data were scaled in Array Star 4 (www.dnastar.com) using Robust Multi-array Average (RMA) analysis with quantile normalization.
|
| |
|
| Submission date |
Oct 23, 2015 |
| Last update date |
Oct 24, 2015 |
| Contact name |
Erin Irish |
| E-mail(s) |
erin-irish@uiowa.edu
|
| Organization name |
University of Iowa
|
| Department |
Biology
|
| Lab |
Irish
|
| Street address |
129 E. Jefferson St.
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL21058 |
| Series (1) |
|
