|
Status |
Public on Jun 21, 2016 |
Title |
Primary AML patient 82 |
Sample type |
RNA |
|
|
Source name |
AML BM
|
Organism |
Homo sapiens |
Characteristics |
tissue: Bone Marrow fab: M2 c/ebpsigma quartile: Q1 karyotypic aberration: t(6;9) DEK/NUP214
|
Extracted molecule |
total RNA |
Extraction protocol |
Total bone marrow RNA was extracted using TRIzol reagent (Invitrogen) according to manufacturer guidelines, quantified using UV spectroscopy (Nanodrop Technologies), and qualitatively assessed using an Agilent2100 Bioanalyzer (Agilent Technologies).
|
Label |
biotin
|
Label protocol |
According to the Affymetrix Whole Transcript (WT) manual, cRNA was prepared from 100 ng total RNA. The cRNA was then used to generate single-stranded DNA, which was fragmented and biotinylated.
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|
|
Hybridization protocol |
Labeled single-stranded DNA in the sense orientation was hybridized for 16 hours at 45 °C on Affymetrix HTA 2.0 microarrays. The microarrays were washed and stained with a streptavidin-phycoerythrin conjugate in an Affymetrix Fluidics Station 450. Signal amplification with antibodies was applied following the instructions provided by Affymetrix.
|
Scan protocol |
The Genechips were scanned with an Affymetrix 7G scanner according to manifacturer guidelines.
|
Description |
Gene expression data from pediatric AML patient according to C/EBPδ expression level (gene-level)
|
Data processing |
The CEL files generated were analyzed through Affymetrix Expression Console Software (version 1.3) which normalizes array signals using a robust multiarray averaging (RMA) algorithm. Normalized data were analyzed using Transcriptome Analysis Console (TAC) 3.0 Software (Affymetrix). An unpaired t-test was applied to identify differentially expressed transcript genes between sample pairs and probes with P-values less than 0.01 and fold-change >2 were declared significant.
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|
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Submission date |
Nov 29, 2015 |
Last update date |
Jun 21, 2016 |
Contact name |
Matteo Zampini |
E-mail(s) |
m.zampini@irpcds.org
|
Phone |
+390498211466
|
Organization name |
Istituto di Ricerca Pediatrica (IRP) Città della Speranza
|
Street address |
corso stati uniti 4
|
City |
Padova |
State/province |
PD |
ZIP/Postal code |
35127 |
Country |
Italy |
|
|
Platform ID |
GPL17586 |
Series (1) |
GSE75461 |
Pediatric AML classification according to C/EBPδ expression |
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