|
| Status |
Public on Feb 01, 2016 |
| Title |
SJ016801 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
non-leukemic bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
tissue: stage-matched bone marrow
|
| Growth protocol |
DNA was extracted from GFP+ leukemic spleen cells derived from C57BL/6 mice transplanted with Arf-/- pre B-cells expressing truncated EPOR ± IK6
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen AllPrep Mini kit
|
| Label |
Cy3
|
| Label protocol |
1.5μg of genomic DNA was labeled using the Agilent ULS Labeling kit
|
| |
|
| Channel 2 |
| Source name |
leukemice cells flow sorted from spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Truncated EPOR + IK6 primary tumor
|
| Growth protocol |
DNA was extracted from GFP+ leukemic spleen cells derived from C57BL/6 mice transplanted with Arf-/- pre B-cells expressing truncated EPOR ± IK6
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen AllPrep Mini kit
|
| Label |
Cy5
|
| Label protocol |
1.5μg of genomic DNA was labeled using the Agilent ULS Labeling kit
|
| |
|
| |
| Hybridization protocol |
Hybridization was carried out in an Agilent oven at 65°C for 40 hours at 20 rpm; after hybridization, slides were washed sequentially according to manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C scanner
|
| Description |
I428-IGH EPOR + Ik6
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.5.1.1) with protocol CGH_105_Jan09 and LOWESS normalization.
|
| |
|
| Submission date |
Dec 16, 2015 |
| Last update date |
Feb 01, 2016 |
| Contact name |
Charles G Mullighan |
| E-mail(s) |
charles.mullighan@stjude.org
|
| Phone |
1-901-595-3387
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Pathology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL10448 |
| Series (1) |
| GSE76061 |
Truncating erythropoietin receptor rearrangements in acute lymphoblastic leukemia |
|
