|
| Status |
Public on Nov 22, 2016 |
| Title |
Oryza sativa OsNPR1 overexpression,biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
G316,the uppermost two internodes
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: the uppermost internodes at heading stage
|
| Treatment protocol |
no treatment
|
| Growth protocol |
the rice plants grow in the paddy filed for about 100 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the two uppermost internodes using the Trizol method (Invitrogen) and purified with RNeasy MiniKit (Qiagen). cDNA was synthesized and purified with one-cycle cDNA Synthesis Kit (Affymetrix) and GeneChip Sample Cleanup Module (Affymetrix), respectively.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol with GeneChip IVT Labeling Kit and Genechip Sample Cleanup Module (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
Gene expression data from the uppermost internodes in rice at heading stage
|
| Data processing |
The data were analyzed with Affymetrix GeneChip® Operating Software (GCOS, Version 1.4) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 18, 2015 |
| Last update date |
Nov 22, 2016 |
| Contact name |
Zuhua He |
| E-mail(s) |
zhhe@sibs.ac.cn
|
| Phone |
86-21-54924121
|
| Organization name |
Chinese Academy of Science
|
| Department |
shanghai institute of plant physiology and ecology
|
| Lab |
He Lab
|
| Street address |
300 Fenglin Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE76178 |
The gene expression data of OsNPR1 overexpression and its wild type Taipei 309 plants. |
|
