|
Status |
Public on Feb 16, 2016 |
Title |
antagomir-132 2 |
Sample type |
RNA |
|
|
Source name |
Renal FoxD1-derivative cells, 10 days after UUO
|
Organism |
Mus musculus |
Characteristics |
tissue: kidney strain: FoxD1-GC;tdTomato sex and age: male age: 8 weeks diagnosis: fibrotic
|
Treatment protocol |
FoxD1-GC;tdTomato were used, antagomir-132 or scramblemir was injected i.v. at 40 mg/kg bodyweight, and unilateral ureter obstruction was performed. 10 days after surgery, mice were sacrificed, kidneys removed and dissociated followed by FACS-mediated isolation of Tomato-positive cells, as described in corresponding manuscript.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with standard Trizol-chloroform protocol.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2
|
Data processing |
The data were normalised using quantile normalisation with Genomestudio
|
|
|
Submission date |
Jan 05, 2016 |
Last update date |
Feb 16, 2016 |
Contact name |
Roel Bijkerk |
E-mail(s) |
r.bijkerk@lumc.nl
|
Organization name |
LUMC
|
Department |
Nephrology
|
Street address |
Albinusdreef 2
|
City |
Leiden |
ZIP/Postal code |
2333 ZA |
Country |
Netherlands |
|
|
Platform ID |
GPL18752 |
Series (2) |
GSE76548 |
miR-132 mediates renal fibrosis: Genome-wide analysis of gene expression in foxd1-derivative interstital cells derived from fibrotic kidneys from mice treated with antagomir-132 or scramblemir |
GSE76550 |
miR-132 mediates renal fibrosis |
|