|
| Status |
Public on Feb 16, 2016 |
| Title |
antagomir-132 2 |
| Sample type |
RNA |
| |
|
| Source name |
Renal FoxD1-derivative cells, 10 days after UUO
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
strain: FoxD1-GC;tdTomato
sex and age: male
age: 8 weeks
diagnosis: fibrotic
|
| Treatment protocol |
FoxD1-GC;tdTomato were used, antagomir-132 or scramblemir was injected i.v. at 40 mg/kg bodyweight, and unilateral ureter obstruction was performed. 10 days after surgery, mice were sacrificed, kidneys removed and dissociated followed by FACS-mediated isolation of Tomato-positive cells, as described in corresponding manuscript.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with standard Trizol-chloroform protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with Genomestudio
|
| |
|
| Submission date |
Jan 05, 2016 |
| Last update date |
Feb 16, 2016 |
| Contact name |
Roel Bijkerk |
| E-mail(s) |
r.bijkerk@lumc.nl
|
| Organization name |
LUMC
|
| Department |
Nephrology
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL18752 |
| Series (2) |
| GSE76548 |
miR-132 mediates renal fibrosis: Genome-wide analysis of gene expression in foxd1-derivative interstital cells derived from fibrotic kidneys from mice treated with antagomir-132 or scramblemir |
| GSE76550 |
miR-132 mediates renal fibrosis |
|
