|
| Status |
Public on Aug 08, 2016 |
| Title |
breast-tum-nit-74-CB |
| Sample type |
RNA |
| |
|
| Source name |
Primary breast tumour, no-intervening treatment, core biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 28
inter-biopsy-interval-(days): 32
grade: 2
biopsy-type: CB
ihc-substype: er
er-allred: 8
pr-allred: NA
her2: 2+fish-ve
size-mm: 20
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction using the RNeasy Mini Kit with RNAse Free DNAse treatment (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated using the IL Encore Biotin Module (NuGEN)
|
| |
|
| Hybridization protocol |
Labelled cDNA was hybridized to Illumina Human HT-12 version 3 and version 4 whole-genome expression bead arrays according to the standard protocol for NuGEN labelled samples
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
X32.CB
|
| Data processing |
The data were normalised using quantile normalisation using lumi R package
|
| |
|
| Submission date |
Jan 11, 2016 |
| Last update date |
Aug 08, 2016 |
| Contact name |
Dominic Andrew Pearce |
| E-mail(s) |
dominic.pearce@ed.ac.uk
|
| Organization name |
The University of Edinburgh
|
| Department |
Institute of Genetics and Molecular Medicine
|
| Lab |
Applied Bioinformatics of Cancer
|
| Street address |
University of Edinburgh Cancer Research UK Centre (ECRC)
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XR |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE76727 |
Tumour sampling method can significantly influence gene expression profiles derived from neoadjuvant window studies II |
| GSE76728 |
Tumour sampling method can significantly influence gene expression profiles derived from neoadjuvant window studies |
|
