|
| Status |
Public on Apr 01, 2016 |
| Title |
Spleen UnInfected_Day 1 KO rep 1_experiment_2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Spleen_UnInfected_Day 1
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6 IFNAR-/-
genotype: IFNAR-/-
gender: Female
tissue: Spleen
treatment: UnInfected_Day 1
experiment: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
9423642104_E
|
| Data processing |
The data were normalised using quantile normalisation with GeneSpring GX12.1
|
| |
|
| Submission date |
Jan 21, 2016 |
| Last update date |
Apr 01, 2016 |
| Contact name |
Krzysztof Potempa |
| E-mail(s) |
potempak@gmail.com
|
| Organization name |
National Institute for Medical Research (NIMR)
|
| Street address |
The Ridgeway
|
| City |
London |
| ZIP/Postal code |
NW7 1AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE77102 |
Analysis of transcriptional signatures in response to Listeria monocytogenes infection reveals temporal and strain dependent changes in interferon signalling |
|
