|
| Status |
Public on Nov 04, 2016 |
| Title |
Control5 |
| Sample type |
RNA |
| |
|
| Source name |
Small intestine, control
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
Sex: male
tissue: proximal small intestine
treatment: control
|
| Treatment protocol |
0.005% Ezetimbe (Ezetrol; Pharmacia, UMCG, Groningen) was added to the lab chow.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TriReagent (Sigma). Quality control was performed with RQS Caliper.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Applied Biosystems).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol using the Illumina iScan.
|
| Description |
6331302016_A
Biological replicate 5
|
| Data processing |
The data were normalized using quantile normalisation strategies improved with a batch controlling Combat algorithm, PMID 20181233.
|
| |
|
| Submission date |
Jan 26, 2016 |
| Last update date |
Nov 04, 2016 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE77236 |
Transintestinal cholesterol transport is important in mice and humans and controls ezetimibe induced fecal neutral sterol excretion |
|
