|
| Status |
Public on Mar 22, 2016 |
| Title |
GSC1 +Dox (I) |
| Sample type |
RNA |
| |
|
| Source name |
CNS tumor spheroid culture, Dox treated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: PDGF-driven glioma spheroid cells (PDGF-GSC)
treatment: Dox
|
| Treatment protocol |
1*10^6 GSCs were plated on 10cm plastic petri dishes and cultured for 48 hours in 10mls of media and then collected for analysis. Cells were either untreated (-Dox) or treated with 100ng/ml Doxycyline dissolved in deionized water. Treatment duration laster for 48 hours before collection.
|
| Growth protocol |
GSCs were grown in DMEM/F12 without L-Glutamine supplemented with 1% Penicillin/Streptomycin and 1X B27 without vitamin A at 37 ͦC in a humidified incubator supplemented with 5%CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Trizol method.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 4
|
| Data processing |
variance stabilizing transformation followed by robust spline normalization, computed by R through the lumi package
|
| |
|
| Submission date |
Jan 29, 2016 |
| Last update date |
Mar 22, 2016 |
| Contact name |
Gilbert Joseph Rahme |
| E-mail(s) |
gilbert.j.rahme@gmail.com
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Cancer Biology
|
| Lab |
Bradley E. Bernstein
|
| Street address |
365 Longwood Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL19441 |
| Series (2) |
| GSE77419 |
PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells. |
| GSE77424 |
PDGF Engages an E2F-USP1 Signaling Pathway to Support ID2-mediated Survival of Proneural Glioma Cells. |
|
