|
| Status |
Public on Aug 13, 2008 |
| Title |
ON_KIDNEY_10 |
| Sample type |
RNA |
| |
|
| Source name |
oncocytoma renal tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
oncocytoma renal tumor tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
R-Phycoerythrin Streptavidin (SAPE), from Molecular Probes, 1 mg/ml ( we use 6 ul per reaction )
|
| Label protocol |
10 ug of Qiagen-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression ratios were obtained by comparing normal human kidney tissue to human kidney tumor tissues. Tumor subtypes include Papillary type 1, and Oncocytoma. Normal and Papillary tissue raw files can be obtained from GSE7023.
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - The version 6 entrez gene ID CDF was used.
|
| |
|
| Submission date |
Jun 25, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL4866 |
| Series (1) |
| GSE8271 |
Chromophobe and Oncocytoma Renal Cell Carcinomas: gene expression and SNP analysis |
|
