|
| Status |
Public on Feb 18, 2016 |
| Title |
Uveal melanoma, PDX model MP042, passage p4 |
| Sample type |
RNA |
| |
|
| Source name |
Patient-Derived Xenograft model
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: PDX
sample origin: Xenograft
models: MP042
tumor type: Primary Tumor
passage: p4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen biopsies using miRNAeasy mini kit (Qiagen, Courtaboeuf, France) recommendations
|
| Label |
biotin
|
| Label protocol |
GeneChip Human Exon 1.0 ST arrays were hybridized according to Affymetrix recommendations using the Ambion WT Expression Kit protocol (Life Technologies) and Affymetrix labeling and hybridization kits
|
| |
|
| Hybridization protocol |
GeneChip Human Exon 1.0 ST microarrays (Affymetrix) were hybridized with 4.7 mg of labeled DNA
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
|
| Description |
Gene expression data from Uveal Melanoma PDX
|
| Data processing |
Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
|
| |
|
| Submission date |
Feb 17, 2016 |
| Last update date |
Feb 18, 2016 |
| Contact name |
Cecile Laurent |
| E-mail(s) |
cecile.laurent@curie.fr
|
| Organization name |
Institut Curie
|
| Street address |
26 rue d'ulm
|
| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
| |
|
| Platform ID |
GPL21472 |
| Series (1) |
| GSE78033 |
Expression Data from Uveal Melanoma patient-derived xenograft and tumor of origin |
|
