|
| Status |
Public on Jan 01, 2008 |
| Title |
Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_1 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic stem cell transfected with siRL
|
| Organism |
Mus musculus |
| Characteristics |
Undifferentiated Dicer -/- ES cell line, passage 22, transfected with siRNA against Renilla luciferase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Absolutely RNA Miniprep Kit (Stratagene)
|
| Label |
biotinylated cRNA
|
| Label protocol |
cRNA was produced by in vitro transcription (IVT) by T7 RNA polymerase, using the Affymetrix IVT kit as per manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
20 µg of biotinylated cRNA was fragmented by heating in the presence of Mg2+ (as per Affymetrix’s instructions) and 15 µg of fragmented cRNA was hybridized to Mouse MOE430 v2.0 GeneChips™
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
ES cells deficient of Dicer_transfected with siRNA agains Renilla luciferase
_date of analysis 01072007
|
| Data processing |
Quality control and background normalization were performed using Refiner from Genedata AG (Basel, Switzerland). All arrays were condensed together using the GC-RMA algorithm with quantile normalization composed of 100 bins using Genedata’s Refiner application. Per chip normalization was performed by scaling the median of the genes called present (detection p-value < 0.04) to a value of 500. Per chip normalized data are referred to as “raw” expression values.
|
| |
|
| Submission date |
Jul 17, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Lasse Sinkkonen |
| E-mail(s) |
lasse.sinkkonen@fmi.ch
|
| Organization name |
Friedrich Miescher Institute
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8503 |
mRNA expression analysis of undifferentiated Dicer -/- (27H10) embryonic stem cells after miRNA transfection |
|
| Relations |
| Reanalyzed by |
GSE119085 |
