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Sample GSM212605 Query DataSets for GSM212605
Status Public on Aug 13, 2007
Title MCF7/c p.1
Sample type RNA
 
Source name Breast Cancer Cell Line
Organism Homo sapiens
Characteristics MCF7 cells stably transfected with pCDNA3.1, passage 1
Biomaterial provider Bianca P. Gomez
Growth protocol Cells were cultured in IMEM supplemented with 5% FBS and 200 ug/ml Geneticin
Extracted molecule total RNA
Extraction protocol Trizol Extraction (Invitrogen), followed by RNeasy Column Cleanup (Qiagen)
Label biotin
Label protocol Standard Protocol for One-Cycle Labeling:

 
Hybridization protocol Standard Protocol for Hybridization:

Scan protocol Standard Protocol for Affymetrix GeneChip Scanner 3000:

Description N/A
Data processing For .CHP file: MAS5.
For .CEL file and manuscript: Robust Multiple-Array Average (RMA) method from the Bioconductor Project.
 
Submission date Jul 23, 2007
Last update date Aug 14, 2011
Contact name Rebecca B. Riggins
E-mail(s) rbr7@georgetown.edu
Phone 202-687-1260
Organization name Georgetown University
Department Oncology, and Lombardi Comprehensive Cancer Center
Street address E412 Research Building, 3970 Reservoir Rd. NW
City Washington
State/province DC
ZIP/Postal code 20057
Country USA
 
Platform ID GPL96
Series (1)
GSE8562 XBP1 confers estrogen independence and antiestrogen resistance in breast cancer cell lines

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 9.37041
1053_at 7.68756
117_at 4.69994
121_at 7.22119
1255_g_at 3.34255
1294_at 5.10215
1316_at 4.22048
1320_at 4.24998
1405_i_at 3.52745
1431_at 3.20539
1438_at 5.50824
1487_at 6.88125
1494_f_at 5.53194
1598_g_at 6.82065
160020_at 6.1267
1729_at 7.15274
177_at 4.46349
1773_at 5.35138
179_at 7.76555
1861_at 6.02552

Total number of rows: 22283

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM212605.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM212605.CHP.gz 120.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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