|
Status |
Public on Aug 13, 2007 |
Title |
MCF7/c p.2 |
Sample type |
RNA |
|
|
Source name |
Breast Cancer Cell Line
|
Organism |
Homo sapiens |
Characteristics |
MCF7 cells stably transfected with pCDNA3.1, passage 2
|
Biomaterial provider |
Bianca P. Gomez
|
Growth protocol |
Cells were cultured in IMEM supplemented with 5% FBS and 200 ug/ml Geneticin
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol Extraction (Invitrogen), followed by RNeasy Column Cleanup (Qiagen)
|
Label |
biotin
|
Label protocol |
Standard Protocol for One-Cycle Labeling:
|
|
|
Hybridization protocol |
Standard Protocol for Hybridization:
|
Scan protocol |
Standard Protocol for Affymetrix GeneChip Scanner 3000:
|
Description |
N/A
|
Data processing |
For .CHP file: MAS5. For .CEL file and manuscript: Robust Multiple-Array Average (RMA) method from the Bioconductor Project.
|
|
|
Submission date |
Jul 23, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Rebecca B. Riggins |
E-mail(s) |
rbr7@georgetown.edu
|
Phone |
202-687-1260
|
Organization name |
Georgetown University
|
Department |
Oncology, and Lombardi Comprehensive Cancer Center
|
Street address |
E412 Research Building, 3970 Reservoir Rd. NW
|
City |
Washington |
State/province |
DC |
ZIP/Postal code |
20057 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE8562 |
XBP1 confers estrogen independence and antiestrogen resistance in breast cancer cell lines |
|