|
| Status |
Public on Aug 13, 2007 |
| Title |
MCF7/c p.2 |
| Sample type |
RNA |
| |
|
| Source name |
Breast Cancer Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
MCF7 cells stably transfected with pCDNA3.1, passage 2
|
| Biomaterial provider |
Bianca P. Gomez
|
| Growth protocol |
Cells were cultured in IMEM supplemented with 5% FBS and 200 ug/ml Geneticin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction (Invitrogen), followed by RNeasy Column Cleanup (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Protocol for One-Cycle Labeling:
|
| |
|
| Hybridization protocol |
Standard Protocol for Hybridization:
|
| Scan protocol |
Standard Protocol for Affymetrix GeneChip Scanner 3000:
|
| Description |
N/A
|
| Data processing |
For .CHP file: MAS5.
For .CEL file and manuscript: Robust Multiple-Array Average (RMA) method from the Bioconductor Project.
|
| |
|
| Submission date |
Jul 23, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Rebecca B. Riggins |
| E-mail(s) |
rbr7@georgetown.edu
|
| Phone |
202-687-1260
|
| Organization name |
Georgetown University
|
| Department |
Oncology, and Lombardi Comprehensive Cancer Center
|
| Street address |
E412 Research Building, 3970 Reservoir Rd. NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20057 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE8562 |
XBP1 confers estrogen independence and antiestrogen resistance in breast cancer cell lines |
|
