|
Status |
Public on Apr 19, 2016 |
Title |
control [14267011831-UH11] |
Sample type |
RNA |
|
|
Source name |
control_mammary tumor
|
Organism |
Mus musculus |
Characteristics |
strain background: FVB gender: female age: 35 weeks tissue: Mammary tumor
|
Treatment protocol |
MMTV-neu Tg mice and SPhK1 KO mice were intercrosserd and MMTV-neu Tg mice with homozygous KO, heterozygous KO and wildtype SphK1 were generated.
|
Growth protocol |
All animals were maintained under controlled conditions of humidity (50±10%), light (12/12 hour light/dark cycle) and temperature (23 ± 2˚C) with free access to feeds and water.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Rneasy Plus kit (QIAGEN) in accordance with prescribed protocol provided with the kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
14267011831-UH11
|
Data processing |
Pre-processing and normalization was done using the Partek microarray data analysis software (http://www.partek.com/). Partek pre-processes raw intensity files from microarray experiment using GCRMA’s background subtraction and uses quantile normalization as the normalization technique.
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|
|
Submission date |
Apr 18, 2016 |
Last update date |
Apr 19, 2016 |
Contact name |
Charles J Rosser |
E-mail(s) |
yoshikos@hawaii.edu
|
Organization name |
University of Hawaii Cancer Center
|
Department |
Clinical and Translational Research Program
|
Lab |
Rosser lab RM 354
|
Street address |
701 Ilalo Street
|
City |
Honolulu |
State/province |
HI |
ZIP/Postal code |
96813 |
Country |
USA |
|
|
Platform ID |
GPL18752 |
Series (1) |
GSE80413 |
Effect of sphingosine kinase 1 deletion on HER/neu-induced mammary tumor development |
|