|
| Status |
Public on Apr 19, 2016 |
| Title |
homozygous KO [14267011834-UH2] |
| Sample type |
RNA |
| |
|
| Source name |
homozygous KO_mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain background: FVB
genotype/variation: MMTV-neu Tg mice with homozygous KO
gender: female
age: 35 weeks
tissue: Mammary tumor
|
| Treatment protocol |
MMTV-neu Tg mice and SPhK1 KO mice were intercrosserd and MMTV-neu Tg mice with homozygous KO, heterozygous KO and wildtype SphK1 were generated.
|
| Growth protocol |
All animals were maintained under controlled conditions of humidity (50±10%), light (12/12 hour light/dark cycle) and temperature (23 ± 2˚C) with free access to feeds and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy Plus kit (QIAGEN) in accordance with prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
14267011834-UH2
|
| Data processing |
Pre-processing and normalization was done using the Partek microarray data analysis software (http://www.partek.com/). Partek pre-processes raw intensity files from microarray experiment using GCRMA’s background subtraction and uses quantile normalization as the normalization technique.
|
| |
|
| Submission date |
Apr 18, 2016 |
| Last update date |
Apr 19, 2016 |
| Contact name |
Charles J Rosser |
| E-mail(s) |
yoshikos@hawaii.edu
|
| Organization name |
University of Hawaii Cancer Center
|
| Department |
Clinical and Translational Research Program
|
| Lab |
Rosser lab RM 354
|
| Street address |
701 Ilalo Street
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96813 |
| Country |
USA |
| |
|
| Platform ID |
GPL18752 |
| Series (1) |
| GSE80413 |
Effect of sphingosine kinase 1 deletion on HER/neu-induced mammary tumor development |
|
