|
| Status |
Public on Jul 27, 2007 |
| Title |
SCCH196 |
| Sample type |
RNA |
| |
|
| Source name |
SCCH196
|
| Organism |
Homo sapiens |
| Characteristics |
EFT cell line expressing EWS/FLI1
|
| Growth protocol |
EFT cells and NB cells were cultured in RPMI 1640 with 10% fetal bovine serum (FBS) at 37°C under a humidified 5% CO2 atmosphere. RMS cells were cultured in MEMa with 10% FBS. UET-13 cells werecultured in Dulbeccoﾕs modified Eagleﾕs medium (DMEM) with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using RNeasy kit (QIAGEN, Valencia, CA) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner GCS3000.
|
| Description |
Gene expression data from a EFT cell line expressing EWS/FLI1 typeI
|
| Data processing |
The arrays were analyzed using GeneChip Operating Software 1.2 (GCOS 1.2) (Affymetrix). Background subtraction and normalization were performed by GeneSpring GX 7.3 software (Agilent Technologies, Inc., Santa Clara, CA).
|
| |
|
| Submission date |
Jul 26, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yoshitaka Miyagawa |
| E-mail(s) |
miyayoshi0007@yahoo.co.jp
|
| Organization name |
National Research Institute for Child Health and Development
|
| Street address |
Setagaya-ku
|
| City |
Okura |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8596 |
Expression profiles of pediatric solid tumor cell lines |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
