|
| Status |
Public on Mar 15, 2017 |
| Title |
HCT8/5Fu-48h-1 |
| Sample type |
RNA |
| |
|
| Source name |
5-FU-induced resistant cell line (HCT8/5- FU) derived from HCT-8 human colorectal cancer (CRC) wild type cells (HCT8/WT), treated with 5-fluorouracil (5-FU) for 48 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT8/5Fu
genotype: 5-FU-induced resistant
|
| Growth protocol |
Medium RPMI-1640 which contains 10% FCS, 1% Penicillin-Streptomycin Solution and 37 °C in a humidified atmosphere containing 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. External spike-in RNAs (ERCC ExFold RNA Spike-In kit, Ambion, 4456739) were added based on cell number. See manuscript for details.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA was prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip 3' IVT Express Kit User Manual, 2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized at 45C for 16 hr at 60RPM on GeneChip Arrays (PrimeView). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the manurfacturer's instructions, using the buffers provided in the Affymetrix GeneChip Hybridization, Wash and Stain Kit.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 and images were extracted with Affymetrix GeneChip Expression Console. A CDF containing the ERCC probes was provided by Affymetrix.
|
| Description |
Gene expression data from 5-FU-induced resistant cell line (HCT8/5- FU) derived from HCT-8 human colorectal cancer (CRC) wild type cells (HCT8/WT), treated with 5-fluorouracil (5-FU) for 48 hours .
HCT85481
|
| Data processing |
The data were analyzed with Bioconductor using the RMA normalization method.
|
| |
|
| Submission date |
May 02, 2016 |
| Last update date |
Mar 15, 2017 |
| Contact name |
Guo Zheng |
| E-mail(s) |
guoz@ems.hrbmu.edu.cn
|
| Organization name |
Fujian Medical University
|
| Department |
Department of Bioinformatics
|
| Lab |
Key Laboratory of Ministry of Education for Gastrointestinal Cancer
|
| Street address |
No.1 Xue Yuan Road
|
| City |
FuZhou |
| State/province |
FuJian |
| ZIP/Postal code |
350000 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE81005 |
Global expression data from HCT-8 human colorectal cancer (CRC) wild type cells (HCT8/WT) and its 5-FU-induced resistant cell line (HCT8/5-FU) |
| GSE81008 |
HCT-8 human colorectal cancer (CRC) wild type cells (HCT8/WT) and its 5-FU-induced resistant cell line (HCT8/5-FU) |
|
