|
| Status |
Public on Feb 19, 2008 |
| Title |
Sez-4 cells starved of IL-2 and activated with IL-2 biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Sez-4 cell line starved of IL-2 and activated with IL-2 biological rep1
|
| Organism |
Homo sapiens |
| Characteristics |
Sez-4 T-cell line was derived from leukemic (Sezary) cells of a patient with a cutaneous T-cell lymphoma
|
| Treatment protocol |
Sez-4 cell line was starved of IL2 for 16h, washed twice and placed into 6-well plates in 10ml RPMI (10% FBS) for 2 h followed by addition of IL-2 (200U), IL-15 (20ng/mL), or IL-21 (100 ng/ml) or medium alone for 4 h.
|
| Growth protocol |
Sez-4 cell line was cultured at 37°C and 5% CO2 in the presence of 200U of IL-2 in standard RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin/fungizone mixture and 2mM L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated using a TRIZOL and Qiagen Rneasy protocols
|
| Label |
biotin
|
| Label protocol |
3ug of input total RNA were amplified and labeled using the Affymetrix 1-cycle kit
|
| |
|
| Hybridization protocol |
according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
|
| Scan protocol |
according to the manufacturer’s instructions as found in GeneChip Expression Analysis Technical Manual, P/N 702232 Rev. 2
|
| Description |
Gene expression profiling of Sez-4 cell line
|
| Data processing |
CEL files and MAS5.0 values with global scaling set to 150 were exported from Affymetrix GCOS (v1.4) software
|
| |
|
| Submission date |
Aug 03, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mariusz Wasik |
| Organization name |
University of Pennsylvania
|
| Department |
Pathology
|
| Street address |
3620 Hamilton Walk
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8685 |
Activation of Sez-4 cell line with IL-2, IL-15 or IL-21. |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
