|
| Status |
Public on Jun 01, 2016 |
| Title |
F1_0006_1 |
| Sample type |
RNA |
| |
|
| Source name |
dorsal skin
|
| Organism |
Mus musculus |
| Characteristics |
hours: 6
genotype: F1
batch: 2
tissue: dorsal skin
treatment: TPA
|
| Treatment protocol |
For TPA treatment, the dorsal skins of FVB/N, Spret/Ei, and FVB/N x Spret/Ei mice were shaved and two days later treated with a single dose of TPA (200 ml of 10-4 M solution in acetone) or vehicle alone. Mice were sacrificed at 0, 2, 4, 6, 24, 48, and 72 hours, and after one week.
|
| Growth protocol |
Mice were aged to 8 weeks, housed in standard conditions with standard chow and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by Qiagen RNEasy clean-up
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
|
| |
|
| Hybridization protocol |
90 microliters of hybridization sample were incubated in the Affymetrix GeneTitan Hybridization Oven instrument for 16 hours at 48C
|
| Scan protocol |
Arrays were processed with the Affymetrix GeneTitan Fluidics Station instrument, using the MoGene-1_1-st-v1-96 fluidics script and GeneTitan Hybridization, Wash, and Stain Kit for WT Array Plates. Arrays were scanned in the Affymetrix GeneTitan Multi-Channel (MC) instrument with the AGCC GeneTitan Instrument Control.
|
| Description |
Gene expression from F1 dorsal skin exposed to TPA
|
| Data processing |
CEL files were background corrected and normalized with RMA using oligo in R and then log2 transformed
|
| |
|
| Submission date |
May 31, 2016 |
| Last update date |
Jun 01, 2016 |
| Contact name |
David Quigley |
| Organization name |
UCSF
|
| Department |
Helen Diller Comprehensive Cancer Center
|
| Lab |
Ashworth Lab
|
| Street address |
1450 Third St. Room 207
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (2) |
| GSE52650 |
Gene expression architecture of mouse dorsal and tail skin reveals functional differences in inflammation and cancer |
| GSE82067 |
Gene expression architecture of mouse dorsal and tail skin reveals functional differences in inflammation and cancer: TPA time course |
|
