|
| Status |
Public on Jun 16, 2016 |
| Title |
miR-206 Mimic, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
RD cells_miR-206 Mimic
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RD
cell type: rhabdomyosarcoma cells
transfected with: miR-206 miRNA Mimic
time point: 48 hrs post transfection
|
| Treatment protocol |
Cells transfected with 30nM miR-206 or NC Mimic with Lipofectamine RNAiMax
|
| Growth protocol |
RD cells grown in 10% FBS, DMEM at 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy extraction of total RNA according to manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Samples were prepared according to Affymetrix protocols (Affymetrix, Inc). 200 nanograms of total RNA was used in conjunction with the Affymetrix recommended protocol for the GeneChip 2.0 ST chips
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials
|
| Scan protocol |
An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
|
| Description |
MEH039
|
| Data processing |
Partek Genomics 6 (Partek Inc., Saint Louis, MO, USA) was used for data processing and analysis.
|
| |
|
| Submission date |
Jun 01, 2016 |
| Last update date |
Jun 17, 2016 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
michael.wang@stjude.org
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE82129 |
PAX7 is a required target for microRNA-206-induced differentiation of fusion-negative rhabdomyosarcoma |
|
