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| Status |
Public on Jun 10, 2016 |
| Title |
miR-25 antagomir RNA seq sample 1 |
| Sample type |
SRA |
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| Source name |
Kidney
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: kidney
age: 9 weeks
treatment: miR-25 antagomir
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells and tissues using TRIzol (Invitrogen, Shanghai, China) according to the manufacturer’s instructions.
A total amount of 1.5 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext RNA Library Prep Kit for Illumina following manufacturer’s recommendations (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using HiSeq 4000 PE Cluster Kit (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 4000 platform (Novogene, Beijing, China) and 150bp paired-end reads were generated
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
AAinh
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| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and mapped to mm9 genome by Tophat2, assembled by cufflink
Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using cufflink and differential expression by cuffdiff
Genome_build: mm9
Supplementary_files_format_and_content: [.txt] tab-delimited text files include FPKM values for each Sample
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| Submission date |
Jun 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Binghai Zhao |
| E-mail(s) |
531581955@qq.com
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| Phone |
8604536585008
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| Organization name |
Mudanjiang Medical University
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| Department |
physiology
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| Lab |
binghaizhao
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| Street address |
No.3 Tongxiang Street
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| City |
Reno |
| State/province |
helongjiang |
| ZIP/Postal code |
157011 |
| Country |
China |
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| Platform ID |
GPL21103 |
| Series (1) |
| GSE83144 |
Alteration in microRNA-25 Balance Causes Heart and Kidney Impairment |
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| Relations |
| BioSample |
SAMN05220585 |
| SRA |
SRX1835342 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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