|
| Status |
Public on Aug 20, 2016 |
| Title |
MM1S_AZD5153_24_1 |
| Sample type |
SRA |
| |
|
| Source name |
Cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line name: MM1S
treatment: AZD5153
treatment time: 24 h
tumor type: mm
replicate: 1
|
| Treatment protocol |
Cell lines were treated with either AZD5153 or DMSO for 24h
|
| Growth protocol |
Cell lines were grown under standard conditions
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA isolation was carried out at Expression Analysis (http://www.q2labsolutions.com/)
Library construction was carried out at Expression Analysis (http://www.q2labsolutions.com/)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
241S_AZD5153_24_1
|
| Data processing |
Reads were soft clipped and provided by the vendor
alignment and analysis were performed using bcbio-nextgen v0.7.7
STAR_2.3.1z_r395 was used for alignment
htseq v0.6.1 was used to calculate counts
FPKM was calculated using Cufflinks v2.1.1
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
|
| |
|
| Submission date |
Aug 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Huawei Chen |
| E-mail(s) |
Raymond.Chen@astrazeneca.com
|
| Organization name |
AstraZeneca
|
| Street address |
35 Gatehouse Dr
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02451 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE85840 |
Transcriptional changes induced by Brd4 inhibitor, AZD5153, in cancer cell lines |
|
| Relations |
| BioSample |
SAMN05597476 |
| SRA |
SRX2035838 |
