|
| Status |
Public on Aug 29, 2018 |
| Title |
Endothelial cells isolated from DNFB-treated ear, NICD1-EC-OE mouse, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
CD31+CD45-Ter119- endothelial cells, FACS-sorted from collagenase I-digested ears.
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ear
cell population: CD31+CD45-Ter119- endothelial cells
genotype/variation: Pdgfb-CreERT2; R26-NICD
|
| Treatment protocol |
Mice were treated with 5 daily tamoxifen injections before the start of experiment. DNFB was applied dermally on two consecutive days (day 0 and 1) to promote sensitisation. Inflammation was elicited by a single application of DNFB to the ear pinna on day 5. Ears were harvested after 24 hours and digested in collagenase I. FACS was performed to isolate CD31+CD45-Ter119- endothelial cells. Cells were sorted directly into Qiagen RLT Plus lysis buffer.
|
| Growth protocol |
NICDiEC-OE mice with endothelial expression of constitutively active Notch1 were generated by combining RosaNICD/NICD with CDH5-iCre transgenics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNEasy Plus Micro kit and amplified using the Affymetrix WT Pico Amplification kit, according to manufacturers description.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 1ng total RNA and amplified according to manufacturers instructions, using the Affymetric GeneChip WT Pico kit.
|
| |
|
| Hybridization protocol |
Following fragmentation and terminal labeling, 5,5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Gene Chip Mouse Gene 2.1 ST array. GeneChips were washed and stained in the Affymetrix GeneCips fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan Multichannel instrument.
|
| Description |
Gene expression data of endothelial cells from DNFB treated ears from mice with tamoxifen-induced endothelial overexpression of constitutively active Notch1
|
| Data processing |
RMA-normalised data were obtained using the Affymetrix Expression Console Software.
|
| |
|
| Submission date |
Aug 24, 2016 |
| Last update date |
Aug 29, 2018 |
| Contact name |
Guttorm Haraldsen |
| E-mail(s) |
guttorm.haraldsen@rr-research.no
|
| Organization name |
Oslo University Hospital
|
| Department |
Department for Pathology
|
| Lab |
LIIPAT
|
| Street address |
Sognsvannsveien 20
|
| City |
OSLO |
| State/province |
OSLO |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE85987 |
Inhibition of endothelial Notch signaling attenuates inflammation |
| GSE85992 |
Gene expression data from endothelial cells isolated from DNFB-treated ears of mice with inducible endothelial-specific overexpression of constitutively active Notch1 intracellular domain |
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