|
| Status |
Public on Mar 01, 2008 |
| Title |
3-Month Epiphycan/Biglycan-deficient-1 |
| Sample type |
RNA |
| |
|
| Source name |
Tibio-femoral joint tissue
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57/Bl6:129 hybrid Gender: male Source: tibio-femoral joint Age: 3 Months Genotype:Epiphycan and Biglycan double-null animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol-mediated organic extraction followed by Qiagen RNeasy column purification as per manufacturer's instruuctions.
|
| Label |
biotin
|
| Label protocol |
Standard in-vitro transcription using biotin-labeled nucleotides (ENZO BioArray High Yield kit)
|
| |
|
| Hybridization protocol |
All hybridizations done as descibed in Affymetrix expression manual.
|
| Scan protocol |
The GeneArray™ Scanner uses an argon-ion laser to excite fluorescent molecules incorporated into the nucleic acid target to generate a quantitative hybridization signal. With precise optics, the GeneArray™ scanner focuses the laser on 3-micron spots within each of the thousands of probe features contained on the GeneChip® probe array. A high-resolution image of the probe array is displayed in real time during scanning, and fluorescence intensity data is automatically stored in a raw file.
|
| Description |
N/A
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Sep 19, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Jason Schultz |
| E-mail(s) |
jschultz@ibt.tamhsc.edu
|
| Phone |
713-677-7580
|
| Organization name |
Texas A&M HSC-IBT
|
| Street address |
2121 W. Holcombe Bvld
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE9099 |
Gene Expression Changes Between 3-Month-Old Epiphycan/Biglycan Double-null and Wildtype Mice |
|
