|
| Status |
Public on Apr 29, 2008 |
| Title |
Glioblastoma tumor GBM_257 |
| Sample type |
RNA |
| |
|
| Source name |
human glioblastoma tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Human glioblastoma tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus2.0. The chips are then transferred to a fluidics instrument that performs washes to remove cRNA that has not hybridized to its complementary oligonucleotide probe. The bound cRNA is then fluorescently labeled using phycoerythrin-conjugated streptavidin (SAPE); additional fluors are then added using biotinylated anti-streptavidin antibody and additional SAPE.
|
| Scan protocol |
Each cRNA bound at its complementary oligonucleotide is excited using a confocal laser scanner, and the positions and intensities of the fluorescent emissions are captured.
|
| Description |
Gene expression data from human glioblastoma tumor.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 26, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yonghong Xiao |
| E-mail(s) |
yonghong_xiao@yahoo.com
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave., HIM218A
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE9171 |
Expression profiles of human glioblastoma frozen tumors and cell lines |
| GSE9200 |
Feedback Circuit among INK4 Tumor Suppressors Constrains Human Glioblastoma Development |
|
| Relations |
| Reanalyzed by |
GSE119087 |
