|
| Status |
Public on Mar 16, 2017 |
| Title |
Bmi1/Mel18-dKO ESC (Constitutive KO) |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic stem cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
antibody: Ring1b antibody (Clone#3)
tissue: Embryonic stem cell
|
| Treatment protocol |
Cells were cultured with complete, cysteine deficient, or valine deficient media with SCF and TPO for 24 hours.
|
| Growth protocol |
ES cells were cultured in standard condition
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using QIAGEN All-prep kit
Library was prepared according to Illumina TruSeq protocol.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
S00260
|
| Data processing |
Basecalls performed using Illumina offline basecaller (OLB) version 1.9.4
Sequences were aligned to mm9 genome using bowtie2 (version 2.1.0) with default parameters.
Genome_build: mm9
Supplementary_files_format_and_content: Wiggle format having RPKM values with 100bp window
|
| |
|
| Submission date |
Sep 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Takaho A. Endo |
| E-mail(s) |
takaho.endo@riken.jp
|
| Organization name |
RIKEN
|
| Department |
IMS
|
| Lab |
Laboratory for Integrative Genomics
|
| Street address |
1-7-22 Suehiro, Tsurumi
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (2) |
| GSE87484 |
PCGF6-PRC1 suppresses premature differentiation of embryonic stem cells by silencing germ cell-related genes [ChIP-Seq] |
| GSE87485 |
PCGF6-PRC1 suppresses premature differentiation of embryonic stem cells by silencing germ cell-related genes |
|
| Relations |
| BioSample |
SAMN05852418 |
| SRA |
SRX2199823 |
