|
| Status |
Public on Dec 20, 2007 |
| Title |
MM0171-002_NspI |
| Sample type |
genomic |
| |
|
| Source name |
lymphoblast or blood
|
| Organism |
Homo sapiens |
| Characteristics |
Family ID: MM0171-002
DNA ID: 47898
Gender: M
Family Type: MPX
Individual Type: parents
|
| Treatment protocol |
Not Applicable
|
| Growth protocol |
Not Applicable
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from blood or lymphoblast using a standard phenol/chloroform method. 500ng of genomic DNA was used for subsequent PCR complexity reduction (GeneChip® Mapping 500K Assay Manual, 2005, Affymetrix)
|
| Label |
biotin
|
| Label protocol |
Biotinylated genomic DNA were prepared according to the standard Affymetrix protocol from 90 ug PCR product (GeneChip® Mapping 500K Assay Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 90 ug of genomic DNA were hybridized for 16 hr at 49C on either the GeneChip® Mapping NspI or StyI chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
SNP data from trio multiplex family
|
| Data processing |
The data were processed using GCOS software. Genotypes were analyzed with GTYPE using Affymetrix defaults.
|
| |
|
| Submission date |
Oct 03, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Christian R. Marshall |
| URL |
http://www.tcag.ca/
|
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics & Genome Biology
|
| Street address |
101 College St.
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3718 |
| Series (1) |
| GSE9222 |
Structural Variation of Chromosomes in Autism Spectrum Disorder. |
|
