|
| Status |
Public on Sep 02, 2009 |
| Title |
Chronic myeloid leukemia CML-#20-BC-250ksty |
| Sample type |
genomic |
| |
|
| Source name |
Blast crisis
|
| Organism |
Homo sapiens |
| Characteristics |
Bone marrow, flow sorted
|
| Treatment protocol |
Samples were obtained at different stages of disease
|
| Growth protocol |
Cryopreserved peripheral blood or bone marrow samples obtained at diagnosis, during disease progression, or after treatment. Blast crisis samples were flow sorted prior to DNA extraction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen DNA blood mini or phenol chloroform
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As per manufacturer (Affymetrix)
|
| Scan protocol |
As per manufacturer (Affymetrix)
|
| Description |
Chronic myeloid leukemia
|
| Data processing |
CEL files and TXT files (generated by Affymetrix GTYPE using the DM algorithm) were processed by dChip and model-based expression performed using the PM/MM model. Normalization was performed using the reference normalization algorithm developed at St Jude and described in Mullighan et al Nature 2007;446:758. The data matrix contains the normalized expression values produced by that algorithm.
|
| |
|
| Submission date |
Oct 10, 2007 |
| Last update date |
Sep 02, 2009 |
| Contact name |
Charles G Mullighan |
| E-mail(s) |
charles.mullighan@stjude.org
|
| Phone |
1-901-595-3387
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Pathology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL3720 |
| Series (2) |
| GSE9110 |
SNP array data for 56 chronic myeloid leukemia samples. |
| GSE9113 |
Affymetrix SNP array data for acute lymphoblastic leukemia samples |
|
