|
| Status |
Public on Oct 01, 2008 |
| Title |
Arabidopsis seedlings (WT) with/without elicitor treatment (color swap) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Chitin oligosaccharide treated Arabidopsis seedlings (WT)
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Arabidopsis seedlings treated with chitinoligosaccharide for 2h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the Arabidopsis seedlings using RNeasy Plant Mini kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
RNA extracts from Arabidopsis seedlings were fluorescence labeled according to the Agilent manufacture’s protocol.
|
| |
|
| Channel 2 |
| Source name |
Arabidopsis seedlings (WT) without elicitor treatment
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Arabidopsis seedlings treated with DW for 2h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the Arabidopsis seedlings using RNeasy Plant Mini kit (QIAGEN).
|
| Label |
Cy5
|
| Label protocol |
RNA extracts from Arabidopsis seedlings were fluorescence labeled according to the Agilent manufacture’s protocol.
|
| |
|
| |
| Hybridization protocol |
The microarrays were hybridized according to the Agilent manufacture’s protocol.
|
| Scan protocol |
The slides were scanned by an Agilent scanner.
|
| Description |
Arabidopsis seeds were surface-sterilized and germinated on the petri plates containing MGRL nutrients supplemented with 0.1% agarose and 1% sucrose. Seedlings were grown for 8-10 days under 16/8 h light/dark cycle at 22ºC (light) or 15ºC (dark). The seedlings were pre-incubated in the fresh MGRL medium in a 48-well microtiter plate for 2 h. One hundred of (GlcNAc)8 was added to the culture medium and incubated for 2h. Total RNA was extracted from the Arabidopsis seedlings using RNeasy Plant Mini kit (QIAGEN).
|
| Data processing |
The obtained results were analyzed using Rosetta Luminator software (Rosetta Inpharmatics LLC).
|
| |
|
| Submission date |
Oct 17, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Hanae Kaku |
| E-mail(s) |
kaku@isc.meiji.ac.jp
|
| Phone |
+81-44-934-7805
|
| Fax |
+81-44-934-7805
|
| Organization name |
Meiji University
|
| Department |
Life Sciences
|
| Street address |
1-1-1, Higashi-Mita, Tama-ku
|
| City |
Kawasaki |
| State/province |
Kanagawa |
| ZIP/Postal code |
214-8571 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2871 |
| Series (1) |
| GSE9346 |
The function of CERK1 receptor-like kinase in the chitin elicitor signaling in Arabidopsis |
|
