|
Status |
Public on Oct 01, 2008 |
Title |
Arabidopsis seedlings (WT) with/without elicitor treatment (color swap) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Chitin oligosaccharide treated Arabidopsis seedlings (WT)
|
Organism |
Arabidopsis thaliana |
Characteristics |
Arabidopsis seedlings treated with chitinoligosaccharide for 2h
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the Arabidopsis seedlings using RNeasy Plant Mini kit (QIAGEN).
|
Label |
Cy3
|
Label protocol |
RNA extracts from Arabidopsis seedlings were fluorescence labeled according to the Agilent manufacture’s protocol.
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|
|
Channel 2 |
Source name |
Arabidopsis seedlings (WT) without elicitor treatment
|
Organism |
Arabidopsis thaliana |
Characteristics |
Arabidopsis seedlings treated with DW for 2h
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the Arabidopsis seedlings using RNeasy Plant Mini kit (QIAGEN).
|
Label |
Cy5
|
Label protocol |
RNA extracts from Arabidopsis seedlings were fluorescence labeled according to the Agilent manufacture’s protocol.
|
|
|
|
Hybridization protocol |
The microarrays were hybridized according to the Agilent manufacture’s protocol.
|
Scan protocol |
The slides were scanned by an Agilent scanner.
|
Description |
Arabidopsis seeds were surface-sterilized and germinated on the petri plates containing MGRL nutrients supplemented with 0.1% agarose and 1% sucrose. Seedlings were grown for 8-10 days under 16/8 h light/dark cycle at 22ºC (light) or 15ºC (dark). The seedlings were pre-incubated in the fresh MGRL medium in a 48-well microtiter plate for 2 h. One hundred of (GlcNAc)8 was added to the culture medium and incubated for 2h. Total RNA was extracted from the Arabidopsis seedlings using RNeasy Plant Mini kit (QIAGEN).
|
Data processing |
The obtained results were analyzed using Rosetta Luminator software (Rosetta Inpharmatics LLC).
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Submission date |
Oct 17, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Hanae Kaku |
E-mail(s) |
kaku@isc.meiji.ac.jp
|
Phone |
+81-44-934-7805
|
Fax |
+81-44-934-7805
|
Organization name |
Meiji University
|
Department |
Life Sciences
|
Street address |
1-1-1, Higashi-Mita, Tama-ku
|
City |
Kawasaki |
State/province |
Kanagawa |
ZIP/Postal code |
214-8571 |
Country |
Japan |
|
|
Platform ID |
GPL2871 |
Series (1) |
GSE9346 |
The function of CERK1 receptor-like kinase in the chitin elicitor signaling in Arabidopsis |
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